Laser Capture Microdissection

Figure 1. Multiple selected regions containing desired specific cell populations for LCM.

For good-quality genome-wide DNA methylation input of a pure population of target cells is required. LCM is an accurate method for isolating these specific target cells out of complex heterogeneous tissue.

For LCM, 16 micron thick sections of FFPE tissue are cut and mounted on membranes fixed on glass slides. The sections are then dewaxed, dehydrated and stained with Hematoxylin. After digital imaging of the stained slides, regions of interest are annotated by a pathologist. The annotated regions are cut by a laser beam using an LCM system and collected in tubes (Figure 2).

After the extraction of genomic DNA from the micro dissected tissues by proteinase K, the concentrations of the extracted DNA are determined, and the samples are ready for MeD-seq analysis.

Figure 2. Isolation of tumor tissue using LCM

Publications about our techniques

Genome-wide DNA methylation profiling using the methylation-dependent restriction enzyme LpnPI Genome Res. 2018 28(1):88-99

MicroRNA expression and DNA methylation profiles do not distinguish between primary and recurrent well-differentiated liposarcoma

Methylation markers FAM19A4 and miR124-2 as triage strategy for primary human papillomavirus screen positive women: A large European multicenter study.

Expression of p16 and HPV E4 on biopsy samples and methylation of FAM19A4 and miR124-2 on cervical cytology samples in the classification of cervical squamous intraepithelial lesions.

Cancer risk stratification of anal intraepithelial neoplasia in HIV-positive men by validated methylation markers associated with progression to cancer.

CADM1 and MAL methylation status in cervical scrapes is representative of the most severe underlying lesion in women with multiple cervical biopsies.

Host Cell Deoxyribonucleic Acid Methylation Markers for the Detection of High-grade Anal Intraepithelial Neoplasia and Anal Cancer.

Reliable identification of women with CIN3+ using hrHPV genotyping and methylation markers in a cytology-screened referral population.

Intra- and inter-laboratory agreement of the FAM19A4/mir124-2 methylation test: Results from an international study.

FAM19A4/miR124-2 methylation in invasive cervical cancer: A retrospective cross-sectional worldwide study.

Defining hrHPV genotypes in cervical intraepithelial neoplasia by laser capture microdissection supports reflex triage of self-samples using HPV16/18 and FAM19A4/miR124-2 methylation.

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